Composite

Part:BBa_K307107:Design

Designed by: Joo-han Gwak   Group: iGEM10_CBNU-Korea   (2010-10-27)

rctA-ori2vc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

using PCR, this part is extracted and amplified.

Source

Vibrio cholerae chromosomeII

References

venkova-canova et al.,"transcriptional inactivation of a regulatory site for replication of Vibrio cholerae chromosomeII",PNAS,Aug8.2006,vol.103,no.32,12051-12056

Elizabeth S.Egan et al.,"Autorepression of RctB, an Initiator of Vibrio cholerae ChromosomeII" Replication,Journal of bacteriology,jan.2006,vol.188,no.2,p.789-793

Debasish Pal, Venkova-canova et al.,"Multipartite Regulation of rctB, the Replication Initiator gene of Vibrio cholerae chromosomeII",Journal of bacteriology,nov.2005,vol.187,no.21,p.7167-7175

stephane Duigou et al.,"Independent control of replication initiation of the two vibrio cholerae chromosomes by DnaA and RctB",journal of bacteriology,sept.2006,vol.188,no.17,p.6419-6424

Anders Lobner-Olesen, "the DNA binding properties of RctB and Replication in vibrio cholerae",Rosklide University Department of Sciencem Systems and Models Microbiology Module2 spring 2007.